IN VITRO EVALUATION OF DIFFERENT CHEMICAL DETOXIFICANTS AGAINST AFLATOXIN (B1&B2) IN COTTON SEED CAKE

Abstract

Four different types of chemical detoxificants namely, Copper sulphate, Benzoic acid, Calcium propionate and Sodium bisulfide were used against 100ug/kg aflatoxin B1(AFB1) and 10ug/kgB2 (AFB2) in cotton seed cake. Four different levels (0.25%, 0.5%, 0.75% and 1.0%) of each of above mentioned chemicals were tested in ELISA technique for detoxification of AFB1 and AFB2infected cotton seed cake. Calcium propionate 0.5% level decreased AFB1from 100ug/kg to 16ug/kg followed by Benzoic acid 0.25% level, which limited AFB1 to 23.3ug/kg. Copper sulphate (0.25%) reduced AFB1 to 25 ug/kg. Other treatments did not remarkably reduce AFB1. Meanwhile Calcium propionate 0.5% level also decreased AFB2.The decrease was from 10 ug/kg to 1.5 ug/kg. Calcium propionate (0.5% level) is quite effective to reduce AFB1andAFB2in cotton seed cake at laboratorial level.