COMPARATIVE EVALUATION OF ANTIBODY TITER AGAINST PASTEURELLA MULTOCIDA IN RABBITS BY USING FOUR DIFFERENT ADJUVANTS IN HS+BQ COMBO VACCINES

Abstract

Hemorrhagic Septicemia (HS) caused by Pasteurella multocida is an economically significant disease of bovines that instigates high monetary losses due to unexpected mortality of animals in emerging countries like Pakistan. Similarly Black Quarter (BQ) caused by Clostridium chauvoei is also an acute infectious ailment of cattle and buffaloes in which severe inflammation of
skeletal and cardiac muscles, severe systemic toxicity and a high mortality occurs. The only way to prevent losses due to both diseases is an effective and long lasting vaccination programme. Four HS+BQ combo vaccines adjuvanted with four different adjuvants were prepared. Two HS+BQ vaccines were prepared by addition of two oil adjuvants i.e Montanide ISA-50V-2 and Eolane-170 while one HS+BQ vaccine was prepared by addition of gel while fourth HS+BQ vaccine was prepared by addition of alum. The bacterial dry weight of P. multocida was adjusted to 2 mg/0.75 mL while the bacterial dry weight for Cl. chauvoei was set at 2.5 mg/0.75 mL. The dose of all vaccines was adjusted to 3 mL /animal. Four groups of rabbits having five rabbits in each group were selected at Veterinary Research Institute, Lahore. From four groups of rabbits one was inoculated with HS+BQ oil based vaccine containing Montanide ISA-50 V2 as adjuvant while other group was inoculated with HS+BQ oil based vaccine containing Eolane-170 oil as adjuvant. Third group was vaccinated with HS+BQ vaccine adjuvanted with gel while fourth group of rabbit was vaccinated with HS+BQ vaccin adjuvanted with alum. All rabbits were vaccinated at dose rate of 1mL / rabbit. Rabbits were again vaccinated with the same vaccines as booster dose after 27 days. Indirect HemAgglutination test was conducted after 141 days intervals on serum samples of all groups. The results showed that both HS+BQ combo oil based vaccines have higher antibody titers as compared to gel and alum based vaccines against HS disease.