BACTERIOLOGICAL AND MYCOLOGICAL ANALYSIS OF MIXED VEGETABLE SALADS

Abstract

Salads are considered healthy food but poor hygienic practices may destroy the quality of salads. The poor quality salads may cause health issues if consumed. A study was carried out to monitor microbiological quality of salads. Samples (n=90) were collected from road side venders, fast food outlets and family restaurants (30/each type), in different localities in Lahore. Samples were processed to determine aerobic bacterial, coliform and fungal counts. The highest mean bacterial count (9.64±2.43), coliform (9.57±2.79) and fungal counts (2.51±2.62) were observed in salads from road side venders. According to the International Commission on Microbiological Specifications for Foods (ICMSF) 73.33 percent of salad samples by bacterial, 98 from coliform and 26.67 by fungal counts were found unacceptable for human consumption. Among isolated bacteria were identified as Staphylococcus aureus, Escherichia coli, Klebsiells, Salmonella, Enterobacter, Aeromonas and Bacillus. The predominant fungal species in salads were Aspergillus fumigatus, Aspergillus niger and Aspergillus flavus. A. fumigatus is an obligate pathogen and Aflatoxin (B1 and B2) produced by A. flavus cause of mycotoxicosis. It was concluded that the salads from road side venders are source of bacteria and fungi having potential for toxins production.