COMPARATIVE EFFICACY OF ORAL FORMULATIONS OF IVERMECTIN AND LEVAMISOLE UNDER IN VITRO CONDITIONS AGAINST HAEMONCHUS CONTORTUS

Abstract

Anthelmintic resistance (AR) is the major constraint towards controlling parasitism of livestock. This study was executed to evaluate relative efficacy of oral formulation consisting of ivermectin (IVM) and levamisole (LEV) towards Haemonchus contortus under in-vitro condition. The worms were isolated from abomasa (n=384), collected from nearby slaughter house. The egg hatch test (EHT), adult motility test (AMT), and larval development test (LDT) were performed for estimation of lethal concentration (Lc) 90. A typical sigmoid dose response was converted to linear function by using Probit transformations. Different concentrations of LEV (0.172, 0.086, 0.043, 0.021, 0.010, 0.005 μl/mL), and IVM (0.002, 0.001, 0.0005, 0.00025, 0.000125, 0.0000625 μl/mL) were incubated with 100 eggs / concentration. The LC 90 values of LEV and IVM were found higher than values suggested by World Association for the Advancement of Veterinary Parasitology (WAAVP), which is (0.1µg/ml). The dose-dependency stipulates development of (AR) anthelmintic towards H. contortus.