ISOLATION AND CHARACTERIZATION OF MAJOR SEED STORAGE PROTEINS: II. APIACEAE FAMILY FOUND IN SINDH, PAKISTAN

Abstract

Seeds of 6 medicinally important plants they belong to family Apiaceae growing in Sindh province of Pakistan, including Anethum graveolens - dill, Apium graveolens - celery, Coriandrum sativum - coriander, Cuminum cyminum - cumin, Foeniculum vulgare -fennel, Trachyspermum ammi L - Carom, the deduction of four main seed storage proteins i.e. albumin, globulin, prolamin and glutelin. All plant species found and cultivated in Sindh, Pakistan. Proteins were seed flour was extracted by sequential steps of extractions including delipidation (removal of oil), water (albumin), 5.0 M NaCl (globulin), 70% ethonal (prolamin), and 0.2 M Na3PO4 buffer, pH8.0 (glutelin). Quantitative estimation was performed Dye binding technique of Bradford used for quantifiable estimation and found huge differences in terms of their concentrations and overall production (Table-1). Among all seed plants the albumin fraction was observed high in family Apiaceae where, C. cyminum (85.01%) and T. ammi L (47.12%) containing the highest, A. graveolans, F. vulgare, C. sativam L (37.66%, 36.88%, 30.14%, respectively) contains the medium while, the lowest concentration was observed in A. graveolans L. (29.11%). Globulin with the second dominant protein fraction may also vary from 3.52% in C. cyminum to 50% in C. sativam L. The meaningful increase in prolamin was observed in T. ammi L (33.08%), A. graveolans L (28.11%), A. graveolans, F. vulgare (21.34%) while, the lowest of around (9.6%) in C. cyminum, C. sativam L seeds. On the other hand, a consistent pattern of 5 to 20% of glutelin concentration was detected among every plant seeds (with exception of A. graveolans L having 23%). To the help of our research information, this study for the first time reported the comparative seed storage proteins profile of the family Apiacea and its possible medicinal and biotechnological application.