IDENTIFICATION OF ANTIFUNGAL COMPOUNDS FROM LEAF EXTRACT OF EUCALYPTUS CITRIODORA AGAINST ASCOCHYTA RABIEI

Abstract

Ascochyta rabiei (Pass.) Lab. is a highly destructive pathogen of chickpea. In this
study, leaf extract of Eucalyptus citriodora was assessed against this pathogen. Bioassays with
methanolic extract (0, 05, 1.0, 1.5, …, 4.0%) extract revealed the remarkable antifungal potential of
leaf extract where 69–94% reduction in biomass of A. rabiei was recorded. Chloroform fraction of this
extract was separated by partitioning the extract in a separating funnel. Using a solvent system of
chloroform: n-hexane (20:80), three compounds were detected on TLC plate which were separated
through preparative TLC and purified on HPLC. GC-MS of the purified compounds lead to the
identification of 3-cyclohexene- 1-ol, 4-methyl-1-(1-methylethyl)- (1), 1-cyclohexene- 1carboxaldehyde,
4-(1-methylethyl)- (2) and eucalyptol (3) that might caused antifungal activity of the extract.