ASSESSMENT OF TRISODIUM CITRATE – VITAMIN C BASED ORAL PREPARATION IN THE TREATMENT OF SUBCLINICAL MASTITIS IN INDIGENOUS ANIMAL (CATTLE, BUFFALO)

Abstract

The major disease that affects dairy industry in Pakistan is mastitis. Mastitis therapy
mostly fails due to pathological changes in udder, pharmacokinetics of drugs used and poor husbandry
practices. The present study is conducted to evaluate non-antibiotic therapy including Trisodium citrate
and Vitamin C for the treatment of subclinical mastitis of cows and buffaloes. The product to be tested
is composed of extemporaneous by mixing the Trisodium citrate 30gm and vitamin C 6gm. A field
trial on therapeutic efficacy of above product has been conducted on buffaloes and cows affected with
subclinical mastitis at Livestock Production Research Institute Bahadurnagar, Okara. Fifteen buffaloes
with 10 animals in treatment group (A1), 5 animals in control group (A2) fifteen cows with 10
animals in treatment group (A3), 5 animals in control group (A4) are use in trial. Surf Field Mastitis
Test (SFMT) is used for grading of animals and treatment is given in both the groups of cattle and
buffalo. Buffalo group A1 and Cattle group A3 shows significantly higher (p<0.05) cure rates. The
milk pH in both the groups decreased up to 6.6. The efficacy of therapeutic protocol was calculated by
Analysis of Variance (ANOVA).