MOLECULAR CHARACTERIZATION, ANTIBIOTIC SUSCEPTIBILITY AND BIOFILM FORMING ABILITY OF PASTEURELLA MULTOCIDA ISOLATED FROM THE RESPIRATORY MICROBIOTA OF HEALTHY BOVINES

Abstract

Livestock plays an important role in the economy of the country because it provides food producing animals. Pasteurella multocida which is an opportunistic pathogen of upper respiratory tract of animals and it has been known to cause many harmful diseases may survive in animal body by forming biofilm in different organs. So, the presence of this bacterium in animals may lead to transfer and threaten the human health by food chain. In the last few years, one of the major problems that alarms and evokes a major horrific threat to human being is the bacterial resistance to antimicrobial agents. Due to deficiency of antimicrobial agents in pipeline and emergence of new mechanisms of intra antimicrobial resistance, many surveillance programs have been set up for its containment. Many factors are responsible for this grave situation like high use of antibiotics in animals. So, the current study concluded that all of the 76 isolates of Pasteurella multocida out of 100 samples were confirmed through PCR and found sensitive to florfenicol (100%), gentamycin (92.11%), chloramphenicol (84.21%) and kanamycin (72.37%) respectively. For tetracycline and neomycin sensitivity were (69.73%) and (64.47%) respectively against the bacteria. Whereas tilmicosin and spectinomycin sensitivity were recorded as (30.27%) and (23.69%). Streptomycin (19.73%), sulfadiazine (7.89%), erythromycin (3.94%) and clindamycin (0.0 %) were least sensitive. It was also concluded that when all pure isolates of Pasteurella multocida were tested for Biofilm assay out of 76 isolates categorized as: non biofilm producer (7.89%), weak biofilm producer (34.21%), moderate biofilm producer (46.05%) and strong biofilm producer (11.84%). Hence, it was concluded that Pasteurella multocida isolated from cattle and buffaloes were found to form biofilm in in-vitro condition.