TY - JOUR AU - A. Javaid, PY - 2023/01/09 Y2 - 2024/03/29 TI - IDENTIFICATION OF ANTIFUNGAL COMPOUNDS FROM LEAF EXTRACT OF EUCALYPTUS CITRIODORA AGAINST ASCOCHYTA RABIEI JF - Pakistan Journal of Science JA - PJS VL - 74 IS - 4 SE - Articles DO - 10.57041/pjs.v74i4.767 UR - https://pjosr.com/index.php/pjs/article/view/767 SP - AB - <p>Ascochyta rabiei (Pass.) Lab. is a highly destructive pathogen of chickpea. In this<br>study, leaf extract of Eucalyptus citriodora was assessed against this pathogen. Bioassays with<br>methanolic extract (0, 05, 1.0, 1.5, …, 4.0%) extract revealed the remarkable antifungal potential of<br>leaf extract where 69–94% reduction in biomass of A. rabiei was recorded. Chloroform fraction of this<br>extract was separated by partitioning the extract in a separating funnel. Using a solvent system of<br>chloroform: n-hexane (20:80), three compounds were detected on TLC plate which were separated<br>through preparative TLC and purified on HPLC. GC-MS of the purified compounds lead to the<br>identification of 3-cyclohexene- 1-ol, 4-methyl-1-(1-methylethyl)- (1), 1-cyclohexene- 1carboxaldehyde,<br>4-(1-methylethyl)- (2) and eucalyptol (3) that might caused antifungal activity of the&nbsp;extract.</p><p>&nbsp;</p> ER -