EFFECTS OF DIFFERENT PARAMETERS ON THE LIPOLYTIC ACTIVITIES OF LIPASE AND PHOSPHOLIPASE EXTRACTED FROM PRUNUS ARMINICA MEAL OF HUNZA CULTIVAR

Abstract

Prunus Arminica.L seeds of Hunza cultivar were defatted with chloroform, methanol mixture (2:1v/v) by ultrasonic assisted extraction technique. The lipase and phospholipase extracted, incubated at 400C and pH 6 in citrate buffer for 1 hour. The lipolytic activities of enzymes were measured at different temperatures i,e 30-700C , pH i,e 3-9 and solvents i,e n-heptane , Di-isopropyl ether and Cyclo hexane. 10 % emulsion of lecithin and olive oil used as the substrate for phospholipase and lipase substrates respectively. The enzymes showed stability at lower temperatures i,e 35-500C and slightly acidic to neutral i.e pH 5 to pH 7. Concentration of liberated fatty acids was the indicator of the enzymes activity which were measured by taking absorbance at 440nm. Stearic acid with different concentrations in hexane and methanol (1:1v/v) were used for the standard curve. Cu-TEA reagent with small quantity of CaCl2 was used for maximum extraction of Cu-FFA and for stable value of blanks in the present study. The enzymes showed maximum activities at 450 C temperature and 6 pH.