DESCRIPTIONS OF ΑLPHA AMYLASE PRODUCED FROM ASPEGILLUS NIGER

Authors

  • F. Siddique Institute of Microbiology, University of agriculture, Faisalabad, Pakistan *Department of Wildlife and Fisheries, Government College University, Faisalabad, Pakistan **Department of Parasitology, University of agriculture, Faisalabad, Pakistan

DOI:

https://doi.org/10.57041/pjs.v66i2.398

Keywords:

Amylase,, , pH, Temperature, Potato,, RiceOptimum.

Abstract

Alpha amylase is one of the most significant enzymes in modern biotechnology. The
aim of this research was to isolate, purify and characterize the strain Aspergillus niger and one of its
enzyme like alpha amylase. For this purpose potato, corn, wheat and rice were used as substrates for
the production of alpha amylase. The optimum conditions for enzyme production were standardized
using various parameters such as substrate, pH and temperature. The results showed that the
production of alpha amylase was highest in case of potato starch in order followed by corn, wheat and
rice starches. Optimum production of enzyme was recorded at 50°C temperature and 6.0 pH during 72
to 96 hours. The maximum activity of enzyme was observed in the presence of calcium chloride as
compared to other metal ions. The study suggested that potato starch could be used as a potential raw
material for the alpha amylase production

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Published

2022-12-29

How to Cite

F. Siddique. (2022). DESCRIPTIONS OF ΑLPHA AMYLASE PRODUCED FROM ASPEGILLUS NIGER. Pakistan Journal of Science, 66(2). https://doi.org/10.57041/pjs.v66i2.398

Issue

Vol. 66 No. 2 (2014): Pakistan Journal of Science

Section

Articles

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Copyright (c) 2014 Pakistan Journal of Science

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