EFFECT OF DIFFERENT PHYTOHORMONES ON PLANT REGENERATION OF AMARYLLIS HIPPEASTRUM

Abstract

Tissue culture of bulbous plants has been successfully established and plantlets are regenerated in large number from this technique. An efficient method for rapid propagation of Amaryllis hippeastrum L. by In Vitro technique has been developed in this study by using micropropagation practice. In this technique explant is inoculated on medium fortified with different
nutrients and growth hormones as well. Scales and meristem of underground bulb used as explants were cultured on MS medium supplemented with different concentrations and combinations of cytokinins and auxins. Effect of these phytohormones on plant formation of Amaryllis hippeastrum was studied. Best results for shoot initiation were obtained by using Thidiazurone (TDZ) 10 µM/l + Alpha-Naphthalene acetic acid (NAA) 1.0 mg/l i.e. (90%) frequency of shoot initiation for scales explants and 80% frequency of shoot initiation for meristems explants with 3.6 ± 0.0632 cm of shoot length for both explants. The best results for multiple shoot production were obtained from the combination of 6-benzyl amino purine (BAP) 5 mg/l + Alpha-Naphthalene acetic acid NAA 0.1 mg/l with 80% frequency of shoot formation Bulblet formation was promoted in MS basal medium fortified with BAP 4 mg/l and NAA 0.1 mg/l NAA + 60% sucrose. The regenerated plantlets were transferred
to different potting media in green house for acclimatization. Best hardening response was obtained in (Vermicompost + Sand). A completely randomized design was used for the experiment. Analysis of data was carried out by using COSTAT V.63 software following Duncan’s New Multiple Range Test.