CLUSTER ANALYSIS IN CHANNA PUNCTATUS FISH REVEALED BY MOLECULAR MARKERS IN PUNJAB-PAKISTAN

Abstract

The studies on genetic diversity of Channa punctatus by using molecular markers
were carried out amongst the different groups of wild populations to investigate the genetic structure
by Randomly Amplified Polymorphic DNA (RAPD) marker and the levels of polymorphism and
similarity. The samples were collected from different five locations viz., Chashma Barrage at River
Indus near District Mianwali, Qadirabad Barrage at Chenab River, Tehsil Wazirabad, District
Gujranwala, Balloki barrage at Ravi River, Tehsil Bhai Pharo, District Kasur, Trimu Barrage at the
junction of Chenab and Jhelum Rivers near District Jhang and Taunsa Barrage at Indus River near
Tehsil Kot Addu District Muzaffar Garh. RAPD data for Jaccard’s coefficient by following the
Unweighted Pair Group Method with Arithmetic Mean (UPGMA) for Hierarchical Clustering of the
similar groups on the basis of similarity amongst the genotypes. The dendrogram divided the randomly
selected individuals of the five populations into four classes/clusters; 17 members in first cluster/class,
4 samples in second cluster/class and 2 samples in the third and fourth class/cluster.