MORPHOLOGICAL CHARACTERIZATION OF IN VITRO SALT TOLERANT CELL LINES AND REGENERATED PLANTS OF DAUCUS CAROTA VAR. SATIVUS

Abstract

The in vitro growth of the plants is affected by different chemical and environmental factors in addition to their genetics. Therefore, the basic requirements of the present study was to standardize the different physical and chemical factors to evaluate the in vitro growth potential of Daucus carota var. sativus under different concentrations of NaCl. For this purpose, Daucus carota was cultured on 1.0 mg/l BAP +0.2 mg/l 2,4-D in MS basal medium. Different concentrations of NaCl were used to evaluate the maximum tolerance of salt stress for carrot in terms of its in vitro growth. It was observed that 0.1 mg/l NaCl stress gave the maximum, i:e 70% growth of carrot in vitro cell lines, at 25±2ºC in 16 hr photoperiod. The data were analyzed statistically through ANOVA technique using SPSS programme.