Production Of Anti-bovine Somatotropin (B-st) Rabbit Polyclonal Antibodies

Abstract

Bovine somatotropin is a growth hormone found in cattle. A commercial preparation of this hormone (bGH) was used as an antigen. Antibodies were raised in rabbit. Primary immunization was done using Freund’s complete adjuvant followed by successive immunizations using Freund’s incomplete adjuvant. The immune response of rabbit was checked by carrying out immune blot assay and qualitative ELISA. Microtiter wells coated with the antigen (bGH) were exposed to primary antibody (rabbit antiserum). The antigen-antibody complex formation was detected by using secondary antibody (sheep anti-rabbit IgG HRP conjugate). The ability of these antibodies to detect bovine growth hormone was also checked by western blotting. Antibodies raised in this study were able to detect bGH in a western blot.