ANTIMICROBIAL AND ANTIOXIDANT SCREENING OF Gardenia tetrasperma

Abstract

Antimicrobial and antioxidant activities of an unexplored medicinal plant, i.e., Gardenia tetrasperma Roxb. was investigated. The antimicrobial potential of extracts of G. tetrasperma was determined using human pathogenic four bacteria and two fungi. The maximum antibacterial potential was recorded, in chloroform extract of leaf, i.e., 91.66±0.4 mm zone of inhibition against E. coli. Chloroform extract of leaves showed 62±1.1 mm zone of inhibition against S. aureus. The methanolic leaf extract showed maximum potential against A.niger and F. solani with the values 42.42±1.5 and 36.22±1.11 mm respectively, whereas the lowest value showed by water extract of the leaf against F.solani, i.e. 16.43±1.37. The methanolic extract of seed also showed satisfactory results against F. solani, i.e. 42±1.75. In seed extracts lowest value was revealed by the water macerates of seeds i.e. 12.33±0.8. The MIC assay was performed for further study which presented the significant MIC value i.e. 0.02±0.1 at 0.8 mg/mL of leaf extract against E. coli where as it was 0.02±0. 9 at 0.2 mg/mL against A. niger. Antioxidant potential was determined using DPPH scavenging potential. The uppermost value of % DPPH was observed as s95.51±1.7 at 500 μL concentration in petroleum ether extract of bark. The maximum values of total antioxidant activity (TAA) were 1.29±0.11 and 1.19±0.5 in methanolic extract of bark and petroleum ether macerates of leaf respectively. Total Phenolic Content (TPC) was 1.702±0.3 and 1.07±3.7 in Petroleum ether macerates of leaf and water macerates of seed, respectively.