EVALUATION OF THE ANTIMICROBIAL AND ANTIOXIDANT PROPERTIES OF Cissampelos pareira L.

Abstract

In present study antimicrobial and antioxidant activities of leaf and stem ethanolic extracts of Cissampelos pareira were evaluated. Stem extract revealed good result as compared to leaf extract. The stem ethanolic extract exhibited good zone of inhibition against Escherichia coli and Pseudomonas aeruginosa with 13±0.33 mm and 14±0.58 mm diameter whereas minimum zone of inhibition was observed by leaf ethanolic extract against Bacillus subtilis and Staphylococcus aureus with 7±0.58 mm and 7±0.88 mm, respectively. The available antibiotics such as tetracycline, cefoperazone and erythromycin were used to compare all these results. The fungal strains i.e. Aspergillus oryzae and Aspergillus niger were used and significant results were recorded for stem ethanolic extract as compared with antifungal standard discs such as terbinafine and fungivin. The MIC assay was used for further analysis of leaf and stem extracts that inhibited bacterial and fungal growth. The antioxidant effect was evaluated by using 1,1-diphenyl-2picrylhydrazyl (DPPH) radical scavenging assay, total phenolic content and total flavonoid content. The stem extract of plant displayed good antioxidant potential as compared to the leaf extract. The % DPPH free radical scavenging activity was maximum of the stem extract (1.03±0.19 μg/ml). The maximum result of antioxidant activity was observed by total phenolic content i.e. 0.77±0.09 μg/ml at 1000 concentration. The ethanolic extract of stem had exhibited most elevated level of flavonoid content i.e. 0.70±0.06 μg/ml as compared to the leaf extract of C. pareira.