IN VITRO ANTIVIRAL ACTIVITY OF STEM AND LEAF EXTRACT OF OLEA FERRUGINEA ROYLE AGAINST FOOT AND MOUTH DISEASE VIRUS

Abstract

The present study was done to discover the natural agent of antiviral that could be essentially and simply utilized in the field, to lessen the animal’s carries condition or to decrease the infection in animals during the outbreak of disease. Culture technique was used to analyze the antiviral or cytotoxic effect of Olea ferruginea Royle against FMD virus. Four kinds of extracts of leaf and stem in polar and non-polar solvents (n-hexane, alcohol, aqueous and chloroform) were used. The extract of n-hexane stem showed toxicity at concentration range of 31.25 μg/mL-125 μg/mL while showed cytotoxicity at 3.9μg/mL to 125μg/mL along with extract showed CSP 54% at 62.5μg/mL or 58% CSP at 125μg/mL concentration, respectively. Cytotoxicity potential of chloroform extract was measured at 500 to 2000 μg/mL which showed that the extract was virucidal at 125μg/mL concentration against FMDV. Likewise, the antiviral significant potential was documented by Olea ferruginea Royle’s alcohol leaf extract at 15.62μg/mL-250μg/mL concentration with 52% to 59% CSP, while the cytotoxic activity of leaf extract was observed at 1000 μg/mL to 2000μg/mL. In the same way, the aqueous leaf extract of Olea ferruginea Royle was observed as virucidal at 62.5μg/mL concentration. In short, Olea ferruginea Royle was used against FMDV due to its antiviral potential.